Western blot analysis of JHDM1D in T-47D cells. Samples were incubated with JHDM1D Polyclonal antibody (Product # PA5-89986) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry-Immunofluorescence analysis of JHDM1D was performed in HCT116 cells using JHDM1D Polyclonal Antibody (Product # PA5-89986) at a dilution of 1:150. Blue: DAPI for nuclear staining.
Supplier Page from Thermo Fisher Scientific for JHDM1D Antibody